欧美熟女人妻一区二区三区_欧美精品精品一区在线_久久福利_天天操天天干天头舔_国产精品久久久久影院嫩草_岛国综合久久_青青草无码国产专区在免费_色综合综合色色综合超碰色天堂_久久久久99精品三人片毛片_国家免费观看一区二区三区四区精品

產(chǎn)品中心您現(xiàn)在的位置：首頁(yè) > 產(chǎn)品展示 > > 特色試劑盒 > CYTO-ID® Autophagy detection kit（1x0.5 µ

CYTO-ID® Autophagy detection kit（1x0.5 µ

更新時(shí)間：2022-01-28

簡(jiǎn)要描述：

CYTO-ID® Autophagy Detection Kit measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic ……

型號(hào)：廠(chǎng)商性質(zhì)：代理商瀏覽量：888

  • No transfection required

  • Proprietary dye includes titratable moieties specific for selectively staining autophagic vesicles

  • Protocol validated with known inhibitors and activators of autophagic activity

  • Rapidly quantifies autophagy in native heterogeneous cell populations

  • Eliminates need for time and effort-consuming transfection efficiency validation required with LC3-GFP transfection

  • Selective and comprehensive staining, allows measurement and differentiation between autophagic flux and autophagolysosome accumulation

  • Negligible staining of lysosomes reduces background seen with other dyes

  • Facilitates high-throughput screening of activators and inhibitors of autophagy
     

CYTO-ID® Autophagy Detection Kit measures autophagic vacuoles and monitors autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles. The dye has been optimized through the identification of titratable functional moieties that allow for minimal staining of lysosomes while exhibiting bright fluorescence upon incorporation into pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes). The assay offers a rapid and quantitative approach to monitoring autophagy in live cells without the need for cell transfection.

Mechanism of Action
The probe is a cationic amphiphilic tracer (CAT) dye that rapidly partitions into cells in a similar manner as drugs that induce phospholipidosis. Careful selection of titratable functional moieties on the dye prevents its accumulation within lysosomes, but enables labeling of vacuoles associated with the autophagy pathway.
CYTO-ID® Autophagy detection kit Fig1-web
Profile autophagy without transfection. Figure 1A: CHO cells stably expressing GFP-LC3 transfected cell lines results in relatively poor baseline separation of control-vs-starved cell populations, making quantification of autophagy difficult. Figure adapted from Shvets E, Fass E, Elazar Z. Figure 1B: The CYTO-ID® Autophagy Detection Kit specifically labels autophagic vacuoles independent of LC3 protein and eliminates the need for transfection. HeLa cells were subjected to starvation and recovery and then labeled with CYTO-ID® Green detection reagent. The dye enables clear detection and quantification of autophagic and pre-autophagic vacuoles that directly correlates to induction of autophagy.
Please mouse over
CYTO-ID® Autophagy detection kit Kit box imageCYTO-ID® Autophagy detection kit Fig3-webCYTO-ID® Autophagy detection kit Fig1-webCYTO-ID® Autophagy detection kit Fig2-webCYTO-ID® Autophagy detection kit Fig4-webCYTO-ID® Autophagy detection kit Fig5-webCYTO-ID® Autophagy detection kit Flow CytometryCYTO-ID® Autophagy detection kit Flow CytometryCYTO-ID® Autophagy detection kit 2.0 image

Product Details

Applications:Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS

Application Notes:The CYTO-ID® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by fluorescence microscopy, flow cytometry, and microplate reader.

Quality Control:A sample from each lot of CYTO-ID® Autophagy detection kit is used to stain HeLa Cells as described in user manual. CYTO-ID® autophagy detection reagent is incorporated into induced cells, observed as accumulative typical spherical vacuoles in foci or throughout cytoplasm. Comparing to untreated HeLa cells, treated sample demonstrate significant increase in fluorescence under microscope.

Quantity:For -K200 size:
200 flow cytometry assays, 250 microscopy assays or 3 x 96-well microplate assays.

For -0050 size:
50 flow cytometry assays, 60 microscopy assays or 1 x 96-well microplate assays.

Use/Stability:With proper storage, the kit components are stable for one year from date of receipt.

Handling:Protect from light. Avoid freeze/thaw cycles.

Shipping:Shipped on Blue Ice

Short Term Storage:-20°C

Long Term Storage:-80°C

Contents:CYTO-ID® Green Detection Reagent
Hoechst 33342 Nuclear Stain
Autophagy Inducer (Rapamycin)
Chloroquine Control
10X Assay Buffer

Scientific Background:Autophagy is a stress-induced protective mechanism. Less active under basal conditions, the mechanism is utilized by eukaryotic cells through lysosome-mediated bulk degradation of cellular contents when subjected to certain hostile conditions such as nutrient depletion and chemical or environmental stress. The role of increased autophagic activity in the pathology of cancer, neurodegeneration, cardiovascular disease and diabetes has become widely recognized and commonly studied. Induction of autophagic flux can be visualized by enhanced accumulation of autophagic vesicles if lysosomal function is inhibited, preventing removal of these vesicles.

Technical Info/Product Notes:The CYTO-ID® Autophagy Detection kit is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications.

Featured in:
Nature Methods - Autophagy: eat thyself, sustain thyself - Nature Methods, 12.2015
Genetic Engineering & Biotechnology News - HTS Profiling Method for Autophagy-Modulators


Application Notes:
Autophagy Analysis Using Object Spot Counting Using Gen5 to Analyze the Size and Number of Autophagosomes Per Nuclei

Towards Understanding the Molecular Basis of Parkinson’s Disease: Cell-based Model of Mitophagy and Aggresome Accumulation

Response Profiles of Known Autophagy-Modulators Across Multiple Cell Lines: Using CYTO-ID® Autophagy Dye to assess Compound Activity and Toxicity

Cell-Based Screening of Focused Bioactive Compound Libraries: Assessing Small Molecule Modulators of the Canonical Wnt Signaling and Autophagy-Lysosome Pathways

A Novel Image-Based Cytometry Method for Autophagy Detection in Living Cells

Predictive High-Content/High-Throughput Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell (iPSC)-Derived Hepatocytes

Visualizing subcellular vesicles to quantitate autophagy in neuronal cells

Cited samples:
For an overview on cited samples please click here.

Protocol:A detailed protocol for FC in primary BMDCs can be found on bioprotocol.org:
Flow Cytometric Analysis of Autophagic Activity with CYTO-ID Staining in Primary Cells by M. Stankov, et al.

Regulatory Status:RUO - Research Use Only


Product Literature References

ABCE1 Regulates RNase L-Induced Autophagy during Viral Infections: B. Ramnan, et al.; Viruses 14, 316 (2021), Abstract; Full Text
Advanced Maternal Age Deteriorates the Developmental Competence of Vitrified Oocytes in Mice: J. H. Lee, et al.; Cells 10, 1563 (2021), Abstract;
Aggregated Tau-PHF6 (VQIVYK) Potentiates NLRP3 Inflammasome Expression and Autophagy in Human Microglial Cells: C. Panda, et al.; Cells 10, 1652 (2021), Abstract;
Alpha-1 antitrypsin counteracts heme-induced endothelial cell inflammatory activation, autophagy dysfunction and death: K. Madyaningrana, et al.; Redox Biol. 46, 10260 (2021), Abstract;
Alpha1-antitrypsin counteracts heme-induced endothelial cell inflammatory activation, autophagy dysfunction and death: K. Madyaningrana, et al.; Redox Biol. 46, 102060 (2021), Abstract;
AMPK-Mediated Metabolic Switching Is High Effective for Phytochemical Levo-Tetrahydropalmatine (l-THP) to Reduce Hepatocellular Carcinoma Tumor Growth: X. Yin, et al.; Metabolites 11, 811 (2021), Abstract;
AMPK-Mediated Metabolic Switching Is High Effective for Phytochemical Levo-Tetrahydropalmatine (l-THP) to Reduce Hepatocellular Carcinoma Tumor Growth: X. Yin, et al.; Metabolites 11, 811 (2021), Abstract;
Anti-Androgen Therapy Radiosensitizes Androgen Receptor Positive Cancers to F-18 Fluorodeoxyglucose: I. Singaravelu, et al.; J. Nucl. Med. 121, 262958 (2021), Abstract;


如需購(gòu)買(mǎi)或咨詢(xún)?cè)摦a(chǎn)品技術(shù)問(wèn)題，請(qǐng)聯(lián)系ENZO一級(jí)代理商-欣博盛生物

留言框

  • 產(chǎn)品：

  • 您的單位：

  • 您的姓名：

  • 聯(lián)系電話(huà)：

  • 常用郵箱：

  • 省份：

  • 詳細(xì)地址：

  • 補(bǔ)充說(shuō)明：

  • 驗(yàn)證碼：

    請(qǐng)輸入計(jì)算結(jié)果（填寫(xiě)阿拉伯?dāng)?shù)字），如：三加四=7

欧美老熟妇操姦视频| 91精品国产色综合久久不卡电影| 久久午夜视频| 国产女人18毛片水真多| 蜜乳AVcOm| 免费精品视频一区二区三区| 91日韩大片| 成人午夜福利视频| 99色色视频| 日本一区二区高清| 精品极品一区| 久久国产一级AV| 99成人福利视频| 超碰欧美精品| 欧美第一页| 午夜高清无码| 中日韩无码精品| 91麻豆精品91久久久久久清纯| 成人熟妇AⅤ集| 91AV综合在线视频| 国产一区二区三区| 欧美性爱视频电影莞式性爱视频电影免费看 | 午夜免费小视频| 91成人网址| 一级片亚洲精品| 欧美久久久久久久久久| 精品WWW| 操的我好舒服的视频国产| 91在线播放视频| 国产免费一区二区三区三州老师F1F1.CC| 大量亚洲自偷自拍| 成人国产片| 国产丝袜一区| 色婷婷Av久久久久久久久久| 色噜噜综合| 成人av一区二区三区| www.丝袜无码| 九色 高清在线| 91热视频色网站| 少妇激情一区二区| 欧美日本黑人轮奸强奸在线一区去视频 |